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Transformace
title: Transformace updated: 2023-12-11T17:19:40 created: 2023-05-25T17:15:20
TOP10 ice
50 ul TOP10 +
5 ul ligate
≤ 1 ul plasmid sol
Ice
Heat shock 42c 40-60 s
Ice + >150 ul LB (rt)
Plate
Pre grow 37’c shaking