Splitting
title: Splitting updated: 2023-11-29T10:47:52 created: 2023-06-02T22:03:21
- High confluance
- WT Trex
- Suck medium, wash with PBS
- Apply trypsin, incubate, detach cells
- Dillute with medium, pipette to falcon
- Centrifuge 250 4 min
- Suck supernatant, resuspend pellet
- Put suspension to new plates
- Usually cca 1/9 for 1 plate