Time and space 1

Complexity of multicellular organism

Feedback loops

• Oscillation - negative feedback - Notch/Dll

• Tissue coordination - interaction of multiple pathways

• Somitogenesis

  • Notch, Wnt, FGF

  • FGF/Erk vs Notch/Hes,her

  • Sync vs out of sync

    • Somites vs brain cells

      Inducers

  • Role of chromatin

    • TAD = topologically associated domain
    • Association - CTCF, cohesins...
    • Chromatin readiness - multiple TFs on multiple sites
    • Example: Hedgehog - fingers, head - tuned by other genes/elements
    • Super enhancers - fast activation of transcription - TFs, chromatin regulators, miRNA - eg stemness factors -