WB

title: WB updated: 2024-01-26T12:23:18 created: 2024-01-10T09:11:58

WB středa 10. ledna 2024 9:11 TREX 2)3

• Lipo2k
• Mirus TransIT 293, Transit pro
• Fugene ?
• Genejet
• Pei

| VDAC1 | Lipo | | --- | --- | | VDAC1 | Mirus | | GFP | Lipo | | GFP | Mirus |

• 500 ng DNA

• 1,25 ul Lipo/1,5 ul miro

• 100 ul opti, 20 min

• 6 hours change medium for lipo cells

• 500 ng dna +1,5 ul fugene GFP fluorescence microscope

• VDAC lysis RIPA + Ppi 150 ul/well

500 ng + 1,5 pei

• 15 min pei + opti
• 20 min pei +dna 500 ng +1,5 genejet
• 15 min gj + dna

100 ul of samples in lawmmli, denatured 98°c with 25 ug protein in 20 ul

Sdspage

• Samples

  | Ladder | Ckia | Lipo | Mirus | Fugene | Pei | Genejet | Notch | Ladder | | | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | | 100 V | | | | | | | | | |

VDAC1 MW = 32 351

CKIA = 37 506

B actin = 42 000

Transfer 15 min 25 v 2.5 a