WB 2

• ~12.9. 12:00 seeded HEK293T to 6well~
• ~13.9 16:00 transfection 2 ug mt/pl/GSK3bK85a/pcdna~
  • ~3:1 PEI~
• 17.9. 15:30 seeded HEK293T to 6wel
• 18.9 14:00 transfect
• 17:00 change med
• 20.9. 13:00
• SDS page samples 20 ug prot