20 ul coip/ 20 ul TCL in laemmli
| | Ladder | | C HA | N1 HA | VDAC HA | N+V HA | V igg | N+V igg | C TCL | N1 TCL | Vdac TCL | N+V TCL | | | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- | --- |
100-115 V 12% acrylamide gel
25v 2.5a 15 min transfer Block 1 h milk rt
Primary 1:1000 1%bsa Ms anti ha Rb anti flag